实习目的：
1.请先熟悉PUBMED的检索界面、检索功能及检索方法。
2.针对一个检索题目，掌握检索步骤与检索策略(特别是检索词与检索式)。
3.完成实习报告
实习要求：1、请同学们独立完成，把答案直接粘贴在问题下面。
2、写出检索入口、检索词、检索式及检索结果的篇数。
PubMed
1.检索我校(注意我校有更名的情况)近5年来被PubMed收录的文献。
步骤：
1.进入pubmed
2. 检索式："Fujian College of Traditional Chinese Medicine"[ad] OR "Fujian University of Traditional Chinese Medicine"[ad]
3. filters出版时间：publication dates=5 years
4.检索结果：132篇
2.请查找近5年发表的有关aristolochic acid(马兜酸)的英文动物实验有免费全文的文献，任选两篇以（summary）的形式下载。
步骤：
1.进入pubmed
2. 检索词：aristolochic acid
3. filters限定languages=English Species=other Animals　 Text availability＝free full text available　 publication dates=5 years
4.检索结果：32篇。
5.任选两篇，设置显示格式为summary。
1: Chen G, Chen H, Wang C, Peng Y, Sun L, Liu H, Liu F. Rapamycin ameliorates
kidney fibrosis by inhibiting the activation of mTOR signaling in interstitial
macrophages and myofibroblasts. PLoS One. 2012;7(3):e33626. Epub 2012 Mar 28.
PubMed PMID: 22470459; PubMed Central PMCID: PMC3314672.
2: Zeng Y, Yang X, Wang J, Fan J, Kong Q, Yu X. Aristolochic acid I induced
autophagy extenuates cell apoptosis via ERK 1/2 pathway in renal tubular
epithelial cells. PLoS One. 2012;7(1):e30312. Epub 2012 Jan 20. PubMed PMID:
22276178; PubMed Central PMCID: PMC3262826.
3. 利用主题词途径查找青蒿素(artemisinin)分离提纯、分析以及药理学研究的文献并任选两篇以abstract 的形式保存，拷贝到文档中。
分析：
主题：青蒿素(artemisinin)　 分离提纯、分析、药理学
策略：主题词/副主题词组配检索方式
步骤：
1.进入mesh database检索界面
2.输入artemisinins
3.勾选副主题词，限定扩展，
检索表达式为："Artemisinins/analysis"[Majr] OR "Artemisinins/isolation and purification"[Majr] OR "Artemisinins/pharmacology"[Majr]
4.检索结果：1056篇
5.选两篇，显示为abstract，保存
1. Pak J Pharm Sci. 2012 Jul;25(3):543-7.
Lamivudine-artesunate co-administration affects glucose metabolism in healthy and
diseased wistar rats.
Olurishe T, Kwanashie H, Anuka J, Muktar H.
Department of Pharmacology & Therapeutics Ahmadu Bello University, Zaria,
Nigeria. olurishe@hotmail.com
HIV-Malaria co morbidity frequently requires the co administration of Lamivudine
and Artesunate, in Malaria endemic areas where HIV is also a problem. This
situation is a frequent occurrence in developing countries of the tropics, like
Nigeria where the burden of Malaria and HIV is heavy. The co administration of
these drugs may result in interactions with possible physiologic and/or
therapeutic consequences. This study investigated the effect of
Lamivudine-Artesunate co administration on body weight, relative organ weight and
glucose tolerance in healthy and diseased (Plasmodium berghei infected and
cyclophosphamide immunosuppressed) wistar rats. Animals received a cumulative 21
day treatment with Lamivudine (20 mg/kg) and/or 7 day Artesunate (10 mg/kg), with
healthy or disease controls. Results showed that organ weights and body weights
were not affected. Oral glucose was however affected in the combination and
Artesunate groups in both disease and healthy rats. The study shows that glucose
tolerance is altered with Lamivudine-Artesunate co administration, and may be
beneficial, as hypoglycaemia is often a complication of Malaria therapy.
PMID: 22713939 [PubMed - indexed for MEDLINE]
2. Biomed Khim. 2012 Jan-Feb;58(1):32-42.
[Soluble guanylate cyclase in the molecular mechanism underlying the therapeutic
action of drugs].
[Article in Russian]
Piatakova NV, Severina IS.
The influence of ambroxol--a mucolytic drug--on the activity of human platelet
soluble guanylate cyclase and rat lung soluble guanylate cyclase and activation
of both enzymes by NO-donors (sodium nitroprusside and Sin-1) were investigated.
Ambroxol in the concentration range from 0.1 to 10 microM had no effect on the
basal activity of both enzymes. Ambroxol inhibited in a concentration-dependent
manner the sodium nitroprusside-induced human platelet soluble guanylate cyclase
and rat lung soluble guanylate cyclase with the IC50 values 3.9 and 2.1 microM,
respectively. Ambroxol did not influence the stimulation of both enzymes by
protoporphyrin IX. The influence of artemisinin--an antimalarial drug--on human
platelet soluble guanylate cyclase activity and the enzyme activation by
NO-donors were investigated. Artemisinin (0.1-100 microM) had no effect on the
basal activity of the enzyme. Artemisinin inhibited in a concentration-dependent
manner the sodium nitroprusside-induced activation of human platelet guanylate
cyclase with an IC50 value 5.6 microM. Artemisinin (10 microM) also inhibited (by
71 +/- 4.0%) the activation of the enzyme by thiol-dependent NO-donor the
derivative of furoxan, 3,4-dicyano-1,2,5-oxadiazolo-2-oxide (10 microM), but did
not influence the stimulation of soluble guanylate cyclase by protoporphyrin IX.
It was concluded that the sygnalling system NO-soluble guanylate cyclase-cGMP is
involved in the molecular mechanism of the therapeutic action of ambroxol and
artemisinin.
PMID: 22642150 [PubMed - indexed for MEDLINE]
4、请查找PUBMED收录福建中医药大学(Fujian University of Traditional Chinese Medicine)吴水生老师的文献，并下载题录。
分析：
福建中医药大学（Fujian University of Traditional Chinese Medicine）
作者＝吴水生
策略：检索字段标识符检索方式
步骤：
1.进入pubmed
2.检索表达式："Fujian University of Traditional Chinese Medicine"[ad] AND "Wu SS"[au]
3.检索结果：1篇
Development of a sensitive and rapid UPLC-MS/MS method for the determination of koumine in rat plasma: application to a pharmacokinetic study.
Chen JZ, Li Y, Xiao JP, Wu SS, Song HW.
Biomed Chromatogr. 2012 Dec 7. doi: 10.1002/bmc.2852. [Epub ahead of print]
PMID: 23225577 [PubMed - as supplied by publisher]
万方医学网
1.请查找美沙拉嗪是不是主题词，如果不是,它对应的主题词是什么,并检索美沙拉嗪治疗应用的相关文献,下载两篇以abstract 的形式保存，拷贝到文档中。
分析：
主题：美沙拉嗪　 治疗应用
能使用中文主题词检索的数据库：万方医学网、中国中医药文献数据库
策略：主题词/副主题词组配检索方式
步骤：
1.进入万方医学网的mesh检索界面
2.检索词：美沙拉嗪
3.检索结果：
美沙拉嗪不是主题词，它对应的主题词是“氨水杨酸”
4. 勾选副主题词，检索表达式为：氨水杨酸/治疗应用
5.检索结果：6篇
6.选择两篇以文摘格式保存。
美沙拉嗪颗粒联合双歧三联活菌片治疗溃疡性结肠炎疗效观察
目的 观察联合美沙拉嗪颗粒和双歧三联活菌片治疗溃疡性结肠炎的临床疗效.方法 选择62例轻、中度结肠炎患者,随机分为治疗组32例,对照组30例.两组均口服美沙拉嗪颗粒1 g, 4次/d,疗程8周,治疗组同时加服双歧三联活菌片4片,3次/d.结果 治疗组完全缓解25例,有效7例,无效0例;对照组完全缓解14例,有效16例.两组相比,差异有统计学意义(P<0.05).结论 联合应用美沙拉嗪颗粒和双歧三联活菌片具有更好的临床疗效.
作 者
马颖光 (郑州大学第五附属医院消化内科,河南,郑州,450052) ； 夏兴洲 (郑州大学第五附属医院消化内科,河南,郑州,450052) ；
刊 名
中国误诊学杂志 2011 年11卷09期 2082-2083页
阿泰宁联合美沙拉嗪治疗溃疡性结肠炎的疗效观察
目的:评价阿泰宁联合美沙拉嗪治疗溃疡性结肠炎临床疗效.方法:对照组25例只给予美沙拉嗪(0.5 g,3次/d),治疗12周.治疗组25例给予阿泰宁(1 260 mg,2次/d)和美沙拉嗪(0.5 g,3次/d),治疗12周.分别统计两组治愈、显效、有效、无效病例数以及总有效率,并对两组总有效率进行样本均数比较的u检验.结果:对照组治愈9例,显效6例,有效4例,无效6例,总有效率为76%.治疗组治愈17例,显效5例,有效2例,无效1例,总有效率为96%.对两组总有效率进行样本均数比较的u检验(P<0.05).结论:阿泰宁联合应用美沙拉嗪比单独应用美沙拉嗪治疗轻中度溃疡性结肠炎更具有临床疗效.
作 者
谭家兴 (辽宁省凤城市中心医院内镜室,118100) ；
刊 名
中国误诊学杂志 2010 年10卷01期 22-23页
2.请查找新药相关的科研简讯、时讯或消息。
分析：
能限定文献类型的数据库：万方医学网、中国中医药文献数据库
步骤：
1.进入万方医学网的论文检索－期刊检索界面
2.检索词：新药
3.勾选相应的文献类型：科研简讯、时讯、消息
5.检索结果：164篇